Composite

Part:BBa_K4494056

Designed by: Shiran Yuan   Group: iGEM22_DKU_China   (2022-09-28)


A. caldus csoR (2022 DKU_China Variant 54)

This part is a variant of Promoter-RBS Combination for A. caldus csoR produced by high-throughput optimization. It is a part of the K4494003-K4494098 collection.

Variant Characterization

Response Curve

Response Curve

Foldchange Value

The "5mM Cu/0 Cu" foldchange value of this part is 0.159094911, ranking No. 84 in our collection.

Evolutionary Distance

The Levenshtein Distance between this part and its original form (K4494196) is 38.

Collection

For more information regarding this collection please check out our wiki.The following shows some important statistical data.

Characterization

Heatmap Characterization

Phylogeny

The following is the minimum-evolution tree of our collection (the numbers are variant order codes):

Mimimum-Evolution Tree

Scatter Plot

The following scatter plot encompasses the foldchange, blank RFU, and Levenshtein Distance to K4494196:

Scatter Plot

Base Pair Count

Unweighted Base Pair Frequency by Position
Unweighted Base Pair Percentages by Position

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 12
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 12
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 12
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 12
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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